Purpose: The purpose of this study was to investigate the relationship of fatigue and Yangsaeng in Korea. Yangsaeng as a traditional health care regimen for the promotion of health and prevention of illness by means specific principles and methods, whose purpose was to improve longevity and healthy life. Method: The subjects of this study were 196 male adults in Korea. Data were collected by using a self-reported questionnaire and analyzed through descriptive statistics, Pearson's correlation coefficient, t-test, ANOVA and multiple linear regression. Results: There was statistically significant difference in Yangsaeng and fatigue according to age and education. The relationship between Yangsaeng and fatigue had a significant negative correlation. Exercise, sleep, mind Yangsaeng were found to be significant predictors (28%) of fatigue. Conclusion: To prevent fatigue of adults, nurse should focus on the factors identified in this study when she develop nursing intervention programs for health promotion.